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gallinae, dsRNA-mediated gene knockdown was rapid, being detectable 24 h after oral delivery of the dsRNA, and persisted for at least 120 h. gallinae resulted in silencing of the targeted gene compared to control mites fed non-specific lacZ dsRNA.
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In addition, feeding Dg vATPase A dsRNA to adult female D. Strikingly, the P-element-induced wimpy testis (PIWI)-interacting RNA (piRNA) pathway was absent in D. gallinae, which indicates that these gene silencing pathways are likely functional. ResultsĬore components of the small interfering RNA (siRNA) and microRNA (miRNA) pathways were identified in D. gallinae vATPase subunit A ( Dg vATPase A) gene and monitor gene knockdown using quantitative PCR (qPCR). In addition we utilise an in vitro feeding device to deliver double-stranded (ds) RNA to D. Here we use an in silico approach to identify core RNAi pathway genes in the recently sequenced D. RNA interference (RNAi)-mediated gene silencing is a valuable tool for studying gene function in non-model organisms, but is also emerging as a novel tool for parasite control. Current acaricide-based controls are unsustainable due to the mite’s ability to rapidly develop resistance, thus developing a novel sustainable means of control for D. Please check the for all current versions of the software.The avian haematophagous ectoparasite Dermanyssus gallinae, commonly known as the poultry red mite, causes significant economic losses to the egg-laying industry worldwide and also represents a significant welfare threat.
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Notes Tucows, Inc has graciously donated a copy of this software to the Internet Archive's Tucows Software Archive for long term preservation and access.
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